In mammals, D-Aspartic acid has been shown to have a significant effect on the hypothalamo-pituitary-testicular axis. In this regard, D-aspartic acid may cause the release of luteinizing hormone from the brain, and also testosterone release from the testes. In rats, D-aspartic acid is found in high concentrations in testicular venous blood, indicating it might be released from the testes. Lower levels of D-aspartic acid are found in other areas of the testes including the extracellular fluid. However, all the areas of the testes have higher concentrations of D-aspartic acid compared with other tissues, indicating that the testes are a significant site of concentration. It is suggested that the secreted D-aspartic acid that enters the testicular venous blood, passes to the rete testicular fluid and then is incorporated into the spermatozoa. This is quite different from the distribution of testosterone, and so it is unclear how D-aspartic acid causes the release of testosterone from the testes. However, evidence today suggests that this amino acid is highly important in male reproductive function with a central role in regulating testosterone in adult males.
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