Bromelain: Pharmacology

Bromelain is the name of a group of endopeptidases of thiol that are found in pineapple (Ananas comosus). The pineapple produces bromelain differently in the stem and fruit and the enzyme products differ chemically, but still fall under the generic name of bromelain. Evidence from studies suggests that bromelain is more abundant in the stem, compared to the fruit. In addition to bromelain, pineapple also contains a number of other peptidase enzymes including ananain and comosain. The palm tree that produces pineapples also produces bromelain in other tissues in smaller amounts, including the roots and leaves. The optimal pH of fruit bromelain is around 6-7 which is more acidic than found in most tissues in humans. In contrast, stem bromelain has an optimal pH of 7-10, suggesting that it may be more active in most human tissues. Bromelain is high in sulphur, containing seven cysteine residues that produce three disulphide bridges. Evidence suggests that bromelain is absorbed reasonably well in humans and once absorbed can bind to two principal antiproteases in the blood called alpha 2-macroglobulin and alpha1-antichymotrypsin.  Maximum blood levels of bromelain occur about 1 hour after oral administration. In one study, after 4 hours roughly 20 % of bromelain remained stable as artificial blood, suggesting that the effects of bromelain may be present for some time. However, if high blood levels are required, supplements should be taken twice per day. 

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Varilla, C., Marcone, M., Paiva, L. and Baptista, J. 2021. Bromelain, a Group of Pineapple Proteolytic Complex Enzymes (Ananas comosus) and Their Possible Therapeutic and Clinical Effects. A Summary. Foods. 1 (10): 2249
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Bromelain: Absorption and Effects on Inflammation

Plant based proteolytic enzymes such as bromelain found in pineapple have been shown to have anti-inflammatory effects. However, these effects are dependent on the enzymes being absorbed into the circulation. Oral administration of pineapple causes an anti-inflammatory effect and so it is assumed that absorption of the bromelain it contains occurs. Studies have indicated that bromelain is absorbed from pineapple, however the absorption rate is low. For example, one study suggested that the maximum absorption level in the blood was 6.3 % following supplemental administration in animals. This suggests that most of the bromelain remains in the gastrointestinal tract, or that extensive metabolism of the bromelain occurs prior to it being absorbed as some other compound. There is a possibility that because it is an enzyme, that the bromelain is digested by the organism’s own proteolytic enzymes in the gut. It is unclear if supplements of bromelain are absorbed differently to bromelain in fruit, but one barrier to absorption for the bromelain in fruit is that the bromelain must be released from the plant cells either through chewing, macerating or heating. 

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Izaka, K., 1972. Gastrointestinal absorption and anti-inflammatory effects of bromelain. Japanese Journal of pHarmacology. 22: 519-534

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Raspberry Ketones: An Anti-Obesity Supplement?

Raspberry ketone is a phytochemical that is extracted from raspberry plants. The raspberry ketone chemical name is 4-(4-hydroxyphenyl) butan-2-one.The ketone has a structure similar to capsaicin and synephrine, two compounds with known weight loss effects. Based on this similar structure, studies have investigated the effects of raspberry ketones on weight loss in mice. For example, in one study mice were fed a high diet with 0.5, 1 or 2 % of raspberry ketones for 10 weeks. In this study the raspberry ketones supplement was significantly able to prevent the high fat diet causing elevations in body weight or the deposition of fat in the liver and visceral adipose tissue. In another study, mice were fed a high fat diet for 6 weeks and then fed the same diet with added 1 % raspberry ketones. In this study the raspberry ketones were effective at reducing the body fat accumulation that was caused by the high fat diet in the initial part of the study. The mechanism of action was observed to be due to increased noradrenaline induced lipolysis that increased translocation of hormone sensitive liaise from the cytosol to lipid droplets in epididymal fat cells. These results suggest that high dose raspberry ketones are effective at causing fat loss in mice. 

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Morimoto, C., Satoh, Y., Hara, M., Inoue, S., Tsujita, T. and Okuda, H. 2005. Anti-obese action of raspberry ketone. Life sciences. 77(2): 194-204
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Bromelain Supplements

Bromelain is a protein digesting enzyme found in pineapple. The purpose of the enzyme is to break down the plant proteins within the cells as a function of the fruit ripening. Other fruits also contain similar enzymes, but in pineapple the enzymes are in particularly high concentrations. Humans can take advantage of bromelain because it has been shown to possess significant anti-inflammatory, antithrombotic and fibrinolytic activity. The anti-inflammatory effects of bromelain relate to its ability to activate some potentially damaging inflammatory mediators, whilst inhibiting others, shifting the balance of inflammation towards a healthy regenerative state. Its ability to break down blood clots and reduce thrombosis and platelet aggregation likely relates directly to its proteolytic activity. Eating pineapple provides a significant amount of bromelain, but this comes with the addition of the sugar in the fruit, and some might not want to eat large amounts of fruit to benefit from the effects of bromelain. Therefore supplements of bromelain are one solution that may provide all of the benefits without any additional energy. 

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RdB

Rathnavelu, V., Alitheen, N.B., Sohila, S., Kanagesan, S. and Ramesh, R., 2016. Potential role of bromelain in clinical and therapeutic applications. Biomedical Reports. 5(3): 283-288
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Nutrition for the Eyes

The eyes are very delicate parts of all animals and as a result they are quite susceptible to disease in humans. Treating eye disorders is difficult and as a result prevention is often the best course of action. As well as making sure that the individual is not too stressed and that they do not strain their eyes, good nutrition is a cornerstone of good eye care. A number of studies have investigated the effects of antioxidants on the health of eyes and found them to be beneficial. Further, the use of vitamin and mineral supplements may also bring health effects to the consumer with regard to eye health. For example a study involving individuals taking 500 mg vitamin C, 400 IU vitamin E, 15 mg beta-carotene, 80 mg zinc (as zinc oxide), and 2 mg copper (as cupric oxide) caused reduction in the relative risk of progression to late-stage age-related macular degeneration from 28% to 20% in 5 years. It should also be mentioned that use of healthy foods in a balanced diet, high in fruits and vegetables would significantly increase eye friendly nutrient intake and has been shown to be beneficial to eye health. 

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Lawrenson, J.G. and Downie, L.E. 2019. Nutrition and eye health. Nutrients. 11(9): 2123
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Pineapple for Ocular Floaters?

Pineapple is an interesting fruit because it contains the proteolytic enzyme bromelain. Bomelaine has some interesting properties as an anti-inflammatory and joint regenerator. Its ability to heal cartilage may relate to the activity of bromelain to break down damaged or dysfunctional cartilage. In terms of its use in eye health, ocular floaters are caused by the breakdown of the vitreous humour of the eye, something that results in small obscurities within the eye that can be seen as small spots floating in the vision. Bromelain may be able to help remove these floaters because its proteolytic activity can break down the floaters which are small pieces of broken off cartilage. For example, in one study researchers used a high dose of bromelain (along with the proteolytic enzymes papain and ficin) to treat patients with vitreous degeneration. The treatment was significantly beneficial at reducing the occurrence of vitreous opacities even in those with the more serious form of the disease (intraocular haemorrhage). The treatment was also well received by the patients who reported positive results from the intervention, suggesting that both objective and subjective measures of the treatment were successful.  

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RdB

Ma, J.W., Hung, J.L., Takeuchi, M., Shieh, P.C. and Horng, C.T. 2022. A New Pharmacological Vitreolysis through the Supplement of Mixed Fruit Enzymes for Patients with Ocular Floaters or Vitreous Haemorrhage-Induced Floaters. Journal of Clinical Medicine. 11(22): 6710
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Preventing Weight Gain Through Conscious Attention

Weight gain is caused by the consumption of the Western diet. This diet is replete with tasty foods that encourage overeating. In particular, high amounts of fat, salt and sugar are added to the mainly processed foods, and this results in a diet that causes significant oxidative damage that then results in downstream consequences to the insulin system such that any energy consumed is more likely to cause weight gain. One solution to this is to make people aware of the problem of weight gain and to intervene with measures aimed at maintaining this self awareness. Such strategies, where individuals are encouraged to focus on their weight through consciously weighing themselves, focusing on their food intakes, as well as equating their food intake with the need to perform physical activity, can be effective at preventing weight gain. For example, in one study researchers found that individuals who were asked to focus more on their weight and how this is affected by other factors were actually less likely to put weight on over the Christmas period compared to those who were not asked to do this. This suggests that consciously being aware of one’s eating habits and trying to focus on the effects of overeating may be enough for some to tip the balance of health away from weight gain, perhaps through small adjustments to eating habits. This suggests that earlier intervention, through education and understanding prior to weight gain, may in some cases prevent the deterioration into metabolic dysfunction and weight gain that characterises many people’s lives. 

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RdB

Mason, F., Farley, A., Pallan, M., Sitch, A., Easter, C. and Daley, A.J. 2018. Effectiveness of a brief behavioural intervention to prevent weight gain over the Christmas holiday period: randomised controlled trial BMJ: 363
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Exercise and Protein Requirements

Animals have a set requirement for the amount of protein they need in their diet, and this relates to a number of factors, most notably the amount of tissue growth and regeneration that is occurring. Exercise is a stress to the body that necessitates the remodeling of skeletal muscle and this is a very energy dependent process. As well as adequate energy in the diet, the body also needs enough protein, and this requirement can be significantly higher than that seen in a sedentary individual. However, it is difficult to state exactly how much protein is required as this will depend on a large number of factors including the biochemical individuality of the person, the type and duration and intensity of exercise undertaken, as well as the quality of the protein. Generally to remodel skeletal muscle, the body prefers to use the branch chained amino acids. Animal proteins containing high amounts of branched chain amino acids, such as whey protein, will therefore allow a more efficient remodeling process. Lower quality proteins that are not complete proteins, such as plant proteins, may be required in higher amounts and may need to be mixed in order to satisfy both the protein requirement and the essential amino acid requirement. Evidence from numerous studies show that high protein diets are not dangerous in any way to healthy humans, and indeed, may actually improve health compared to high carbohydrate diets. 

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Lemon, P.W. 1998. Effects of exercise on dietary protein requirements. International journal of sport nutrition. 8: 426-447
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A Little Sauce?

Many people choose to add condiments to their food to enhance the taste. The first thing to note about this is that if there is a requirement to add taste to food, it is likely that the food has not been prepared well and is perhaps of low quality. Most healthy foods, if approached from a perspective of a neutral taste that has not been blunted by the Western diet, are actually fairly tasty when some effort is made to prepare them. The second point to note about sauces, is that in many cases these contain some ingredients that may not be considered healthy. In particular, many sauces are high in added sugar, salt and preservatives. In itself this may not be an issue as the amount eaten may be small, but the problem with adding such sauces is that they can increase the addictiveness of the food and encourage overeating. That they often encourage overeating of junk foods makes matters worse. There are some exceptions to this, most notably apples sauce, which is often added to pork. Apple sauce is essentially mashed up apples, and it therefore retains many of the health properties of the original fruit (minus the peel). Mint sauce may also provide significant health benefits because of the mint it contains, the only other ingredient being vinegar, which is also a health food. Following on from this, vinegar added to food may also have beneficial glycaemic effects. 

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Yeast Spreads and Mood Disorders

Yeast spreads are foods that are derived from the brewing industry, and which are designed to be added to bread and toast. Two of the most common brands include Vegemite and Marmite, but most supermarkets sell their own branded versions. These products use the waste products of the brewing industry to create a dark coloured spread that is very high in B vitamins. Often the products are fortified with additional B vitamins. A deficiency of B vitamins has been shown to increase the risk of mood disorders including forms of anxiety and depression. Studies have suggested that eating yeast spreads may reduce the risk of anxiety in some individuals, and this effect is likely most evident in those who have anxiety caused by low levels of vitamins. In particular, B vitamins have been shown to help regulate stress levels and intakes of B vitamins in those who are stressed may have significant benefits. As stress is a primary cause of anxiety, this may explain the benefit of yeast spreads. Yeast spreads can therefore act as a significant source of B vitamins and may have specific benefits on the mental health of the consumer. 

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RdB

Mikkelsen, K., Hallam, K., Stojanovska, L. and Apostolopoulos, V.  2018. Yeast based spreads improve anxiety and stress. Journal of functional foods. 40. 471-476
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